Mutation Detection

Richard G. H. Cotton (Autor)

Buch | Softcover

216 Seiten

1996
Oxford University Press (Verlag)
978-0-19-854889-8 (ISBN)

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Fundamental to the study of cancer is the ability to detect mutations. This book aims to critically review the field. It refers to methodology and protocols, gives illustrative examples and puts the whole emerging field in context. The question of ethics is also discussed.

Cancer and the mechanisms of its development are receiving increasing research interest. New genes are being isolated almost daily, Fundamental to those studies is the ability to detect mutations. Mutation detection is time consuming and expensive and thus, should be undertaken with a thorough knowledge of the alternative methods. This book aims to critically review the field, refer to methodology and protocols and give illustrative examples as well as put the whole
emerging field in context including ethics.

1. Introduction ; 2. Sequencing Methods ; 3. Scanning Methods ; 3.1 Physical Methods ; 3.1.1. Denaturing Gradient Gel Electrophoresis ; 3.1.2. Single Strand Conformational Polymorphism ; 3.1.3. Heteroduplex ; 3.2. Mismatch Detection/Cleavage ; 3.2.1. Ribonuclease ; 3.2.2. Chemical Cleavage ; 3.2.3. Carbodiimide ; 3.2.3. DNA endonucleases and binding proteins ; 3.3. Other ; 4.1. Allele Specific Oligonucleotide ; 4.2. Ligase and LCR ; 4.3. Allele Specific Amplification ; 4.4. Primer Extension ; 4.5. Artificial Introduction of Restriction Sites ; 4.6. Restriction Enzymes ; 5. Associated Methodology ; 5.1. Southern Blots ; 5.2. PCR ; ILLEGITIMATE TRANSCRIPTION ; 5.4. Gene Specific Strategies ; 6. Current Weaknesses and Future Requirements

Zusatzinfo	halftone and line illustrations, tables
Verlagsort	Oxford
Sprache	englisch
Maße	156 x 233 mm
Gewicht	405 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Studium ► 2. Studienabschnitt (Klinik) ► Anamnese / Körperliche Untersuchung
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-19-854889-3 / 0198548893
ISBN-13	978-0-19-854889-8 / 9780198548898
Zustand	Neuware