Detection of Cell Death Mechanisms

Determination of Caspase Activation by Western Blot.- Detection of Unfolded Protein Response by Polymerase Chain Reaction.- Subcellular Fractionation to Demonstrate Activation of Intrinsic Apoptotic Pathway.- A Triple-Parameter Based Laboratory Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells.- Flow Cytometric Analyses of p53-mediated Cell Cycle Arrest and Apoptosis in Cancer Cells.- A Real-Time, Bioluminescent Apoptosis Assay.- Detection of Anoikis Using Cell Viability Dye and Quantitation of Caspase Activity.- Time and Dose Dependent Toxicity Studies in 3D Cultures Using a Luminescent Lactate Dehydrogenase Assay.- Visualization and Quantification of Neutrophil Extracellular Traps.- 2In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps.- Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture.- Visualization of Necroptotic Cell Death through Transmission Electron Microscopy.- Methodology for Comprehensive Detection of Pyroptosis.- Determination and Quantitation of Cytotoxic T Cell-Mediated Cell Death.- Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model.- A Luminescent Assay to Quantify Cell Viability in Real Time.- Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis.- Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes.- Determining Cell Death Pathway and Regulation by Enrichment Analysis.- Transcription Factor Binding Site Identification and Enrichment Analysis.