Arabidopsis Protocols

Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses.- Bioinformatic Tools in Arabidopsis Research.- Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene.- Non-sterile Grafting Methods for Arabidopsis.- CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana.- Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration.- Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts.- Photosynthetic and Heterotrophic Cell Suspension Cultures.- Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals.- Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana.- Identification and Quantification of Small RNAs.- Flow Cytometry and Sorting in Arabidopsis.- Live Imaging ofArabidopsis Leaf and Vegetative Meristem Development.- Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging.- Using Tripartite Split-sfGFP for the Study of Membrane Protein–Protein Interactions.- High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling.- Atomic Force Microscopy to Study Cell Wall Mechanics in Plants.- Identification and Characterization of Reproductive Mutations in Arabidopsis.- Metabolomic Analysis of Natural Variation in Arabidopsis.- Untargeted Metabolomics of Arabidopsis Stomatal Immunity.- Biotin-based Proximity Labeling Of Protein Complexes in Planta.- Phosphoproteomic Analysis of Plant Membranes.- RNA-binding Protein Immunoprecipitation and High Throughput Sequencing.