Plant Endosomes

Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis.- Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.- Analysis of Endoplasmic Reticulum-Endosomes Association Using Live-Cell Imaging In Plant Cells.- Degradation of Abscisic Acid Receptors through the Endosomal Pathway.- Biochemical and Imaging Analysis of ALIX Function in the Endosomal Trafficking of Arabidopsis Protein Cargoes.- Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network.- Imaging Plant Cells by High-pressure Freezing and Serial Block-Face Scanning Electron Microscopy.- Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking.- Assessing Extrinsic Membrane Protein Dependency to PI4P using a Plasma Membrane to Endosome Re-localisation Transient Assay in Nicotiana benthamiana.- Subcellular Localization of PI3P in Arabidopsis.- Immuno-purification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis.- Cell-free Protein Translation System For Expression Of Lipid-binding Proteins Tagged with Small Epitopes and their use in Protein-lipid Overlay Assays.- Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants.- Detection of Phosphorylation on Immunoprecipitates from Total Protein Extracts of Arabidopsis thaliana Seedlings.- Purification and Interaction Analysis of a Plant-specific RAB5 Effector by in vitro pull-down Assay.