New Methods and Sensors for Membrane and Cell Volume Research

Irena Levitan, PhD, is a Professor of Medicine and Bioengineering at the University of Illinois at Chicago. She received her PhD in Biophysics and Neurobiology at the Hebrew University of Jerusalem in 1994 and completed postdoctoral training at the Medical College of Pennsylvania and Institute for Medicine and Engineering at the University of Pennsylvania. Her research focuses on the biophysical properties of endothelial membranes and sub-membrane cytoskeleton. Specifically, the studies of her group, which combine computational and experimental biophysical approaches, provided the first comprehensive structural insights into cholesterol regulation of K+ channels. In 2012, she was named a Guyton Distinguished Lecturer “for her quantitative and biophysical work on cholesterol modulation of ion channels and how this can affect integrated organ function”. She and her group also discovered a paradoxical relationship between fluidity/deformability of the membrane and cell stiffness. In 2018, she was elected a Fellow of AIMBE for “outstanding contributions to our understanding of lipid-ion channel interactions, cellular biomechanics and vascular dysfunction under dyslipidemia”. Michael Model got an M.S. in materials science from Russia and continued his education at the University of Michigan, where he received a Ph.D. in biophysics in 1995. Since 2004, he has been working at Kent State University, first as a microscopy facility manager and currently as an associate professor in the Department of Biological Sciences. While at Kent State, he developed a new microscopic method to measure cell volume, and that prompted him to turn to the physiology of cell volume regulation. He is also interested in the biological role and control of macromolecular crowding and continues to work on microscopic techniques and their applications.

On, in, and under membrane
 M.M. Model and I. Levitan
1. Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell
Luca Mantovanelli, Bauke F. Gaastra and Bert Poolman

2. Current methods for studying intracellular liquid-liquid phase separation
Amber R Titus and Edgar E Kooijman

3. Investigating molecular crowding during cell division in budding yeast with FRET
Sarah Lecinski, Jack W. Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald and Mark C. Leake

4. The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC
Yulia Kolobkova, Sumaira Pervaiz and Tobias Stauber

5. Studying cell volume beyond cell volume
Michael A. Model

6. Membrane tension
Pei-Chuan Chao and Frederick Sachs

7. Methods for assessment of membrane protrusion dynamics
Jordan Fauser, Martin Brennan, Denis Tsygankov and Andrei V. Karginov

8. Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids
Irena Levitan

9. Fluorescence sensors for imaging membrane lipid domains and cholesterol
Francisco J. Barrantes

10. Mass spectrometry-based lipid analysis and imaging
Koralege C. Pathmasiri, Thu T.A. Nguyen, Nigina Khamidova and Stephanie M. Cologna

11. Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering
Ursula Perez-Salas, Yuri Gerelli, Lionel Porcar and Sumit Garg