Epidermal Cells
Springer-Verlag New York Inc.
978-1-0716-0253-9 (ISBN)
Comprehensive and cutting-edge, Epidermal Cells: Methods and Protocols, Fourth Edition serves as a valuable reference for experts as well as novices in the epidermal cell field.
The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse.- Dermal-Epidermal Separation by Heat.- Dermal-Epidermal Separation by Enzyme.- Dermal-Epidermal Separation by Chemical.- Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures.- Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential.- Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis.- Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte.- Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry.- Keratinocyte Differentiation by Flow Cytometry.- In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis.- Genetic Modification of Human Primary Keratinoctes by Lentiviral Vectors.- Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9.- In Vitro Wound Healing Assays to Investigate Epidermal Migration.- Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells.- Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse.- Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures.- Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data.- Cell-Extracellular Matrix Adhesion Assay.- Methods for Analysis of Keratinocyte Migration.- Scratch Wound Healing Assay.- Photoprotective Activity Assay toward Ultraviolet B in Human Keratinocytes.- Photodynamic Therapy Assay.- A Method to Prepare Claudin-Modulating Recombinant Proteins.- Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application.- Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media.
Erscheinungsdatum | 19.03.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2109 |
Zusatzinfo | 43 Illustrations, color; 14 Illustrations, black and white; XII, 301 p. 57 illus., 43 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Cell Culture • Dermal cells • Keratinocytes • Regenerative medicine • Skin Cells |
ISBN-10 | 1-0716-0253-5 / 1071602535 |
ISBN-13 | 978-1-0716-0253-9 / 9781071602539 |
Zustand | Neuware |
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