Cell Cycle Oscillators

Cell Cycle Control: A System of Interlinking Oscillators.- Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity.- Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts.- Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts.- Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry.- Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots.- Construction of a Full-Length 3’UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs.- Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis.- Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block.- Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition.- Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System.- EDU (5-Ethynyl-2′-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle.- High-Resolution Analysis of Centrosome Behavior During Mitosis.- Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells.- Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle.- Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell Cycle Indicator (Fucci).- Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody.- Analyzing Centrioles and Cilia by Expansion Microscopy.- Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae.- Application of PALM Super Resolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans.- Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools.- Calorimetric Heat Dissipation Measurements of Developing Zebrafish Embryos.- The Conditional Knockout Analogous System: CRISPR-Mediated Knock Out Together with Inducible Degron and Transcription-Controlled Expression.