Expressed Protein Ligation
Springer-Verlag New York Inc.
978-1-0716-0436-6 (ISBN)
Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Methods and Applications of Expressed Protein Ligation.- Protein Splicing: From the Foundations to the Development of Biotechnological Applications.- Identification, Characterization, and Optimization of Split Inteins.- Methods to Study the Structure and Catalytic Activity of cis-splicing Inteins.- Expressed Protein Ligation: General Experimental Protocols.- Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins.- Native Chemical Ligation Via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid Phase Peptide Synthesis.-Fused Split Inteins: Tools for Introducing Multiple Protein Modifications.- Streamlined Expressed Protein Ligation: Site-specific Antibody-drug Conjugate.- In vivo Histone Labeling Using Ultrafast trans-Splicing Inteins.- Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation.- Site-specific Phosphorylation of PDZ Domains.- Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications.- Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol.- Investigating the Effects of O-GlcNAc Modifications in Parkinson’s Disease using Semi-synthetic α-synuclein.- Recombinant Expression of Cyclotides Using Expressed Protein Ligation.- Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines.
Erscheinungsdatum | 16.03.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2133 |
Zusatzinfo | 67 Illustrations, color; 28 Illustrations, black and white; X, 362 p. 95 illus., 67 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | C-terminal thioesters • hydrazide oxidation • Protein Engineering • Protein Splicing • split inteins |
ISBN-10 | 1-0716-0436-8 / 1071604368 |
ISBN-13 | 978-1-0716-0436-6 / 9781071604366 |
Zustand | Neuware |
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