Purinergic Signaling

Introduction to Purinergic Signaling.- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling.- Agonists and Antagonists for Purinergic Receptors.- Homology Modelling of P2X Receptors.- Using RNA Interference for Purinoceptor Knock Down in vivo.- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos.- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues.- Flow Cytometry of Membrane Purinoreceptors.- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation.- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking.- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer.- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy.- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor.- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse.- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging.- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels .- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader.- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes.- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells.- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages.- Automated Planar Patch-clamp Recording of P2X Receptors.- Controlling Engineered P2X Receptors with Light.- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment.- Assays to Measure Purinoceptor Pore Dilation.- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization.- Measuring Leukocyte Migration to Nucleotides.- Assessment of Cell Adhesion after Purinoceptor Activation.