Purinergic Signaling
Humana Press Inc. (Verlag)
978-1-4939-9716-9 (ISBN)
Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide asound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.
Introduction to Purinergic Signaling.- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling.- Agonists and Antagonists for Purinergic Receptors.- Homology Modelling of P2X Receptors.- Using RNA Interference for Purinoceptor Knock Down in vivo.- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos.- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues.- Flow Cytometry of Membrane Purinoreceptors.- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation.- Multimeric Ionotropic Purinoceptor Detection by Protein Cross-linking.- Multimeric Purinoceptor Detection by Bioluminiscence Resonance Energy Transfer.- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy.- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor.- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse.- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging.- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels .- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader.- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes.- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells.- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages.- Automated Planar Patch-clamp Recording of P2X Receptors.- Controlling Engineered P2X Receptors with Light.- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment.- Assays to Measure Purinoceptor Pore Dilation.- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization.- Measuring Leukocyte Migration to Nucleotides.- Assessment of Cell Adhesion after Purinoceptor Activation.
Erscheinungsdatum | 08.11.2019 |
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Reihe/Serie | Methods in Molecular Biology ; 2041 |
Zusatzinfo | 49 Illustrations, color; 33 Illustrations, black and white; XII, 360 p. 82 illus., 49 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Animal Models • drug targeting • nucleotides • Pharmacology development • Purinergic receptors |
ISBN-10 | 1-4939-9716-5 / 1493997165 |
ISBN-13 | 978-1-4939-9716-9 / 9781493997169 |
Zustand | Neuware |
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