Molecular Methods in Plant Biology

Krishnananda Pralhad Ingle obtained his PhD in Agricultural Biotechnology from Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India. He is an Associate Scientist, Jain Irrigation Systems Ltd., Jalgaon. He also served as an editorial board member of Acta Scientific Journal of Agriculture (International) and Science Publishing Group (USA). He has a vast experience in the Molecular Breeding and Biotechnological aspects. He has worked for esteem organizations like MAHYCO, India Ltd. (Jalna); VNR seeds Pvt. Ltd. (Raipur); and Biostadt MH Seeds Ltd. (Hyderabad). He has published 5 articles in magazines and newspapers and 28 national and international publications with topics spanning a wide range from DNA profiling methodology to plant molecular systems, phytochemistry, entomological aspects. Mangesh Pradip Moharil is Associate Professor of Biotechnology, Department of Agricultural Botany, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India. Dr. Moharil obtained his PhD (2006) from the Sant Gadge Baba Amravati University, Amravati, Maharashtra, India. He has submitted 5 Gene Bank accessions at NCBI, USA. He is a member of the Society of Biotechnologist (India), and Microbiologist Society (Maharashtra), India. He has a vast experience in the field of Molecular Biology and has 14 years of teaching experience in the area of Molecular Biology, Genomics and Proteomics, Microbial Biotechnology, Genomics and Proteomics, and Entomological Research. He has published 16 international and 25 national papers and total 51 research publications till date with a wide range from Molecular Biology, Plant Genomics, Mutation Breeding, Molecular Breeding, Molecular Marker system and Plant Genomics. Dipika Ashok Padole is Junior Research Assistant, Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India. She obtained her PhD from the Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India. She has a vast experience in the field of microbial study, insect molecular biotechnology, genetic transformation studies, and plant tissue culture. She has submitted Gene Bank accessions for Helicoverpa chitin synthase B gene at NCBI, USA. She has a vast experience in the Plant Tissue Culture and other biotechnological aspects. Dr. Dipika has published total 16 national and international publications with a wide range of topics from microbial, phytochemistry, entomological aspects to genetic transformation studies.

1. Isolation of Plant Genomic DNA
2. Isolation of Plasmid DNA
3. Polyacrylamide Gel Electrophoresis
4. Polymerase Chain Reaction (PCR) Amplification
5. RNA Isolation Using TRIzol Method
6. Expression Analysis Using Semi-Quantitative Real Time-PCR
7. Quantitative Expression Analysis by Real Time-PCR
8. Analysis of DNA Sequences by Southern Blotting and Hybridization
9. Analysis of DNA Sequences by Northern Blotting and Hybridization
10. Histochemical GUS Assay
11. Enzyme-Linked Immunosorbent Assay (ELISA)
12. DNA Gel Extraction Protocol
13. Competent Cell Preparation
14. Ligation, Transformation and Colony PCR
15. DNA Sequencing and Analysis
Glossary
Instruments Used in the Laboratory
Appendix