Phytoplasmas

Phytoplasmas: An Introduction.- Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques.- Phytoplasma Transmission: Insect Rearing and Infection Protocols.- Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors.- Symptoms of Phytoplasma Diseases.- Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas.- Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene.- PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas.- Real-time PCR Protocol for Phytoplasma Detection and Quantification.- Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves.- A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas.- One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas.- A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification.- Quantitative Analysis with Droplet Digital PCR.- Rapid Sample Preparation and LAMP for Phytoplasma Detection.- Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly.- Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of ‘Flavescence Dorée’.- Transcriptomic Analyses of Phytoplasmas.- Transcriptomic Analyses of Phytoplasmas.- Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study.- Collection of Phloem Sap in Phytoplasma-infected Plants.- DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas.- Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas.- Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC).- Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants.- Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants.