Cytoskeleton Dynamics

Purification of Mammalian Tubulins and Tubulin-Associated Proteins using a P2A-based Expression System.- Expression and Purification of Microtubule Associated Proteins from HEK293T Cells for In Vitro Reconstitution.- In vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule Severing Enzymes.- In vitro Microtubule Dynamics Assays using Darkfield Microscopy.- In vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Laments in Emulsion Droplets.- Studying Tau-microtubule Interaction using Single Molecule TIRF Microscopy.- In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis.- Methods for Studying Membrane-associated Bacterial Cytoskeleton Proteins in vivo by TIRF Microscopy.- Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-like Assemblies.- Studying Centriole Duplication and Elongation in Human Cells.- Assaying Microtubule Nucleation.- Detection of Microtubule Nucleation Hotspots at the Golgi.- Ultrastructural Analysis of Microtubule Ends.- Optogenetic Control of Microtubule Dynamics.- Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin.- Functional Dissection of Mitosis using Immortalized Fibroblasts from the Indian Muntjac, A Placental Mammal with only Three Chromosomes.- Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes.- Using the Four-cell C. elegans Embryo to Study Contractile Ring Dynamics during Cytokinesis.- Knocking out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifications.- Measuring the Role of Tubulin Posttranslational Modifications on Axonal Transport.- Methods to Measure Perinuclear Actin Dynamics during Nuclear Movement in Migrating Cells.