Two-Hybrid Systems

Protein Interactomics by Two-Hybrid Methods.- Making the Right Choice: Critical Parameters of the Y2H Systems.- Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening.- Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System.- The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions.- A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning.- Analyses of Protein Interaction Networks Using Computational Tools.- Options and Considerations When Using a Yeast One-Hybrid System.- Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins.- Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens.- Transcription Factor-Centered Yeast One-Hybrid Assay.- The Yeast Three-Hybrid System for Protein Interactions.- The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants.- Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules.- Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System.- Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells.- Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology.- KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions.- Coimmunoprecipitation of Interacting Proteins in Plants.- Analysis of Protein-Protein Interaction by Co-IP in Human Cells.- Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures.- Transient Transactivation Studies in Nicotiana benthamiana Leaves.- The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions.- Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells.