Macrophages

Culture of Human Monocyte-Derived Macrophages.- A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages.- Growing Murine Bone Marrow-Derived Macrophages.- Immortalization of Murine Bone Marrow-Derived Macrophages.- Isolation and Identification of Murine Serous Cavity Macrophages.- Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies.- Isolation and Phenotyping of Adult Mouse Microglial Cells.- Isolation and Phenotyping of Bone Marrow Macrophages.- Isolation and Phenotyping of Intestinal Macrophages.- Phenoytyping Tumor Associated Macrophages.- Activating Murine Macrophages In Vitro.- Polarizing Macrophages In Vitro.- Viral Replication Assay in Bone Marrow-Derived Macrophages.- Macrophage Bactericidal Assays.- Quantitative Phagocytosis Assays in Primary and Cultured Macrophages.- Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM).- Chromatine Immunoprecipitation in Macrophages.- Transfecting Macrophages.- In Vitro Migration Assays.- Cytokine-Induced Acute Inflammatory Monarticular Arthritis.- Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections.- Genetic Models of Macrophage Depletion.- Depleting Macrophages In Vivo with Clodronate-Liposomes.- Cre Driver Mice Targeting Macrophages.