Embryonic Stem Cell Protocols

Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition.- A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.- Resolving Heterogeneity: Fluorescence Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture.- Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium.- Generation of Embryonic Stem Cells in Rabbits.- Gene Transfer into Pluripotent Stem Cells Via Lentiviral Transduction.- Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells.- Expanding the Utility of FUCCI Reporters Using FACS-Based ‘Omics Analysis.- Selection of Antibodies Interfering with Cell-Surface Receptor Signalling Using Embryonic Stem Cell Differentiation.- Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells.- Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells.- Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells.- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation.- Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers.- Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells.- From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells.- In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor’s Production.- Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes.- Intracellular Calcium Measurements for Functional Characterization ofNeuronal Phenotypes.- In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets.- Generation of Corneal Keratocytes from Human Embryonic Stem Cells.- Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells.- Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool.- Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis.- 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells.- A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells.- Applying Shear Stress to Pluripotent Stem Cells.- Generating Inner Ear Organoids from Mouse Embryonic Stem Cells.- Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells.- Pancreatic Differentiation from Murine Embryonic Stem Cells.- Osteogenic Differentiation from Embryonic Stem Cells.- Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells.