Serum/Plasma Proteomics
Humana Press Inc. (Verlag)
978-1-4939-8380-3 (ISBN)
Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.
Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation.- A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma.- A Proteomic Protocol for the Preparation of Platelet Concentrates.- Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture.- Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma.- Characterization of the Low-molecular Weight Human Plasma Peptidome.- In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion.- Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry.- Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins.- Identification of Core-fucosylated Glycoproteome in Human Plasma.- Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis.- Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein.- Red Blood Cells in Clinical Proteomics.- High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS.- Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers.- Lipidomics of Human Blood Plasma by High Resolution Shotgun Mass Spectrometry.- Proteomics Analysis of Circulating Serum Exosomes.- High-density Serum/Plasma Reverse Phase Protein Arrays.- Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis.- Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody.- Serum Profiling for Identification of Autoantibody Signatures in Diseases using Protein Microarrays.- Quantitative Comparisons of Large Numbers of Human Plasma Samples using TMT10plex Labelling.- Efficient Quantitative Comparisons of Plasma Proteomes using Label-free Analysis with MaxQuant.- Blood and Plasma Proteomics: Targeted Quantitation and Post-translational Redox Modifications.- SWATH Mass Spectrometry for Proteomics of Non-depleted Plasma.- Shotgun and Targeted Plasma Proteomics to Predict Prognosis of Non-Small Cell Lung Cancer.- High-throughput Parallel Proteomic Sample Preparation using 96-well Polyvinylidene Fluoride (PVDF) Membranes and C18 Purification Plates.- Targeted Quantification of the Glycated Peptides of Human Serum Albumin.- Absolute Quantification of Middle to High Abundant Plasma Proteins via Targeted Proteomics.- A Highly Automated Shotgun Proteomic Workflow – Clinical Scale and Robustness for Biomarker Discovery in Blood.- Mass Spectrometry-based Serum Proteomics for Biomarker Discovery and Validation.- Metabolomics Towards Biomarker Discovery.- Plasma Biomarker Identification and Quantification by Microparticle Proteomics.- Bronchoalveolar Lavage Quantitative Mass Spectrometry based Proteomics Analysis in Lung Diseases.- Protein Multiplexed Immunoassay Analysis with R.- Appendix I Standard operating procedures for plasma collection in clinical research.- Appendix II Standard operating procedures for serum collection in clinical research.- Appendix III Reference ranges for blood tests.
Erscheinungsdatum | 29.10.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1619 |
Zusatzinfo | 70 Illustrations, color; 24 Illustrations, black and white; XVI, 551 p. 94 illus., 70 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
ISBN-10 | 1-4939-8380-6 / 1493983806 |
ISBN-13 | 978-1-4939-8380-3 / 9781493983803 |
Zustand | Neuware |
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