Selenoproteins -

Selenoproteins

Methods and Protocols

Laurent Chavatte (Herausgeber)

Buch | Softcover
340 Seiten
2018 | Softcover reprint of the original 1st ed. 2018
Humana Press Inc. (Verlag)
978-1-4939-8433-6 (ISBN)
171,19 inkl. MwSt
This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.

SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins.- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins.- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly.- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing.- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs.- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis.- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling.- Modification of Selenoprotein mRNAs by Cap Tri-methylation.- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC – ICP MS).-Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum.- Radioactive 75Se Labeling and Detection of Selenoproteins.- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines.- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips.- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues.- Overexpression of Recombinant Selenoproteins in E. coli.- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS.- Selenocysteine Mediated Expressed Protein Ligation of SELENOM.- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity.- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities.- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk.- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis. 

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1661
Zusatzinfo 35 Illustrations, color; 28 Illustrations, black and white; XV, 340 p. 63 illus., 35 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
ISBN-10 1-4939-8433-0 / 1493984330
ISBN-13 978-1-4939-8433-6 / 9781493984336
Zustand Neuware
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