Selenoproteins

SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins.- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins.- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly.- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing.- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs.- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis.- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling.- Modification of Selenoprotein mRNAs by Cap Tri-methylation.- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC – ICP MS).-Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum.- Radioactive 75Se Labeling and Detection of Selenoproteins.- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines.- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips.- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues.- Overexpression of Recombinant Selenoproteins in E. coli.- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS.- Selenocysteine Mediated Expressed Protein Ligation of SELENOM.- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity.- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities.- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk.- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis.