The Hippo Pathway

The Power of Drosophila Genetics: The Discovery of the Hippo Pathway.- Drosophila Genetics: The Power of Genetic Mosaic Approaches.- Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues.- Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs.- Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence.- Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions.- Immunohistochemistry to Study YAP in Human Tissue Samples.-Immunofluorescence Study of Endogenous YAP in Mammalian Cells.- Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells.- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors.- Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells.- Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells.- HTRF® Total and Phospho-YAP(Ser127) Cellular Assays.- Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids.- Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview.- CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels.- Hippo Pathway Regulation by Tyrosine Kinases.- Structural and Biochemical Analyses of the Core Components of the Hippo Pathway.- Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro.- GST Pull-Down Assay to Measure Complex Formations.- Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag.- Quantifying the Kinase Activities of MST1/2.- Measuring the Kinase Activities of the LATS/NDR Protein Kinases.- MST1/2 Kinase Assays Using Recombinant Proteins.- Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development.- The Hippo Signaling Pathway in Regenerative Medicine.