SUMO
Humana Press Inc. (Verlag)
978-1-4939-8175-5 (ISBN)
Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.
Concepts and Methodologies to Study Protein SUMOylation: An Overview.- The Regulation of Chromatin by Dynamic SUMO Modifications.- Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex.- Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria.- A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO Conjugation.- Identification and Characterization of SUMO-SIM Interactions.- Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties.- Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins.- In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific Proteases.- Detection of Protein SUMOylation In Situ by Proximity Ligation Assays.- In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized Cells.- Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO Strategy.- Label-Free Identification and Quantification of SUMO Target Proteins.- The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteins.- Isolation of In Vivo SUMOylated Chromatin-Bound Proteins.- Identification of Substrates of Protein-Group SUMOylation.- Tools to Study SUMO Conjugation in Caenorhabditis elegans.- Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry Analysis.- Detection of SUMOylation in Plasmodium falciparum.- Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.
Erscheinungsdatum | 03.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1475 |
Zusatzinfo | 34 Illustrations, color; 20 Illustrations, black and white; XI, 306 p. 54 illus., 34 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Schlagworte | Cell Cultures • chromatin regulation • enzymes • Model organisms • Protein modification • small ubiquitin-related modifier SUMO • SUMO molecules |
ISBN-10 | 1-4939-8175-7 / 1493981757 |
ISBN-13 | 978-1-4939-8175-5 / 9781493981755 |
Zustand | Neuware |
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