Chromosome and Genomic Engineering in Plants
Humana Press Inc. (Verlag)
978-1-4939-8163-2 (ISBN)
Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.
Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences.- Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase.- Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking.- Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants.- One-Step Generation of Chromosomal Rearrangements in Rice.- Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana.- Gametocidal Systems for Dissecting Wheat Chromosomes.- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases.- Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 System.- Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination.- Development of Genome Engineering Tools fromPlant-Specific PPR Proteins Using Animal Cultured Cells.- Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes.- Image Analysis of DNA Fiber and Nucleus in Plants.- Detection of Transgenes on DNA Fibers.- Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems.- Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes.- Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements.
Erscheinungsdatum | 03.08.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1469 |
Zusatzinfo | 38 Illustrations, color; 24 Illustrations, black and white; XI, 218 p. 62 illus., 38 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Schlagworte | Ac/DS system • Cre/LoxP • DNA insertion • Genome editing • plant artificial chromosomes • plant chromosome vector • plant genetics & genomics |
ISBN-10 | 1-4939-8163-3 / 1493981633 |
ISBN-13 | 978-1-4939-8163-2 / 9781493981632 |
Zustand | Neuware |
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