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Flow Cytometry

A Practical Approach

M.G. Ormerod (Herausgeber)

Buch | Softcover
305 Seiten
1994 | 2nd Revised edition
Oxford University Press (Verlag)
978-0-19-963461-3 (ISBN)
37,35 inkl. MwSt
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An updated edition of a laboratory manual which aims to provide biologists with an introduction to the relatively new technique of flow cytometry. The text contains the contributions of leading experts in the field, who offer advice and guidance on this specialized form of microscopy.
This is an updated edition of a laboratory manual which aims to provide biologists with an introduction to this relatively new technique. Leading experts offer valuable advice and guidance on this specialized form of microscopy, which is fast developing as an important application in modern hospital laboratories and biological and medical research laboratories. It should be beneficial to researchers in cell biology and biochemistry who already use flow cytometry, or who wish to use it for the first time.

Introduction to the principles of flow cytometry; An introduction to fluorescence technology; Preparing suspensions of single cells; Flow sorting; Immunofluorescence of surface markers; Measurement of cytoplasmic and nuclear antigens; Analysis of DNA - general methods; Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method; Analysis of cell proliferation using the bromodeoxyuridine-Hoechst ethidium bromide method; Chromosome analysis and sorting; Intracellular ionized calcium, magnesium, membrane potential and pH; Flow cytometry of RNA content; Measurement of dynamic cellular events; Further applications to cell biology.

Erscheint lt. Verlag 1.7.1994
Reihe/Serie A Practical Approach ; No.142
Zusatzinfo halftones, numerous line figures, tables, bibliography
Verlagsort Oxford
Sprache englisch
Maße 160 x 230 mm
Gewicht 550 g
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 0-19-963461-0 / 0199634610
ISBN-13 978-0-19-963461-3 / 9780199634613
Zustand Neuware
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