Flow Cytometry -

Flow Cytometry

A Practical Approach

Michael Ormerod (Herausgeber)

Buch | Softcover
296 Seiten
2000 | 3rd Revised edition
Oxford University Press (Verlag)
978-0-19-963824-6 (ISBN)
89,95 inkl. MwSt
Flow cytometry is a technique widely used in biological research and in diagnostic medicine. Flow cytometers are found in most biological research institutions and most clinical laboratories in larger hospitals.
Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

Introduction to the principles of flow cytometry ; Fluorescence and fluorescence technology ; Preparing of suspensions of single cells ; Flow sorting ; Immunofluorescence of surface markers ; Analysis of DNA - general methods ; Further clinical applications ; Quality control in the clinical laboratory ; Measurement of cytoplasmic and nuclear antigens ; Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method ; Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method ; Chromosome analysis and sorting by flow cytometry ; Intracellular ionised calcium, magnesium, membrane potential and pH ; Flow cytometry in the study of apoptosis ; Further applications to cell and molecular biology

Erscheint lt. Verlag 18.5.2000
Reihe/Serie Practical Approach Series ; 229
Zusatzinfo numerous figures
Verlagsort Oxford
Sprache englisch
Maße 188 x 245 mm
Gewicht 580 g
Themenwelt Studium 1. Studienabschnitt (Vorklinik) Histologie / Embryologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 0-19-963824-1 / 0199638241
ISBN-13 978-0-19-963824-6 / 9780199638246
Zustand Neuware
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