Oxidative Folding of Peptides and Proteins (eBook)

Johannes Buchner is based in the Department of Chemistry at the Technical University of Munich. His research interests focus on protein folding in vivo and in vitro, molecular chaperones, folding catalysts, antibody production and engineering. He is author and co-author of 150 publications including the Protein Folding Handbook. Luis Moroder is based at the Max Planck Institute. His research interests focus on biorganic chemistry, peptide chemistry, structure-function studies of bioactive peptides, conformational analysis and protein engineering. He is author and co-author of over 500 publications.

Preface. Foreword. Chapter 1. Oxidative Folding of Proteins in Vivo: Thioredoxins and the regulation of redox conditions in prokaryotes; Dsb A B; Eucaryotic PDIs; Structure of Ero1/oxidation in the ER; Oxidative folding in the ER; Oxidative protein folding in mitochondria; Cellular responses to redox stress; Harnessing disulfide bond formation in the periplasm of bacteria for recombinant protein production. Chapter 2. Oxidative Folding of Proteins in vitro: The role of disulfide bonds for folding and stability of proteins; Strategies for the oxidative refolding of disulfide-bonded proteins. Chapter 3. Redox potentials of cysteine residues in peptides and proteins: Methods for their determination. Chapter 4. Engineering disulfide bonds. Chapter 5. Selenocysteine as a probe of oxidative protein folding. Chapter 6. Oxidative Folding of Peptides in vitro: Oxidative folding of single-stranded disulfide-rich Peptides; Regioselective disulfide formation; Folding motifs of cysteine-rich peptides; Double-stranded cysteine-peptides; Multiple-strand cysteine-peptides. Chapter 7. Cysteine-based scaffolds for functional miniature proteins. Chapter 8. Selenocystine-peptides - Synthesis, folding and applications.