Oxidative Folding of Peptides and Proteins

Johannes Buchner is based in the Department of Chemistry at the Technical University of Munich. His research interests focus on protein folding in vivo and in vitro, molecular chaperones, folding catalysts, antibody production and engineering. He is author and co-author of 150 publications including the Protein Folding Handbook. Luis Moroder is based at the Max Planck Institute. His research interests focus on biorganic chemistry, peptide chemistry, structure-function studies of bioactive peptides, conformational analysis and protein engineering. He is author and co-author of over 500 publications.

Foreword;

Preface;

Oxidative Folding of Proteins in vivo;

Disulfide-bond Formation and Isomerization in Prokaryotes;

The Periplasm of E. coli - Oxidative Folding of Recombinant Proteins;

Oxidative Protein Folding in Mitochondria;

Oxidative Folding in the Endoplasmic Reticulum;

The Ero1 Sulfhydryl Oxidase and the Oxidizing Potential of the Endoplasmic Reticulum;

Eukaryotic Protein Disulfide-isomerases and their Potential in the Production of Disulfide-bonded Protein Products: What We Need to Know but Do Not!;

Cellular Responses to Oxidative Stress;

Oxidative Folding of Proteins in vitro;

Strategies for the Oxidative in vitro Refolding of Disulfide-bridge-containing Proteins;

Redox Potentials of Cysteine Residues in Peptides and Proteins: Methods for their Determination;

Engineered Disulfide Bonds for Protein Design;

Selenocysteine as a Probe of Oxidative Protein Folding;

Oxidative Folding of Peptides in vitro;

Regioselective Disulfide Formation;

Folding Motifs of Cystine-rich Peptides;

Double-stranded Cystine Peptides;

Multiple-strand Cystine Peptides;

Cystine-based Scaffolds for Functional Miniature Proteins;

Selenocystine Peptides – Synthesis, Folding and Applications;

Subject Index