Schwann Cells

Isolation of Schwann Cell Precursors from Rodents.- Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.- Isolation and Expansion of Schwann Cells from Transgenic Mouse Models.- Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers.- Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells.- Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations.- Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies.- Chemical Conversion of Human Fibroblasts into functional Schwann Cells.- Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats.- Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells.- Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay.- Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.- Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis.-  Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures.- The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes.- In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia.- A Culture Model To Study Neuron – Schwann cell – Astrocyte Interactions.- Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development.- Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs.- 3D Cancer Migration Assay with Schwann Cells.- Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis.- Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration.- The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin.- Analysis of Myelinating Schwann Cells in Human Skin Biopsies.- Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae.- Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin.- Live Imaging of Schwann Cell Development in Zebra Fish.- Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord.- Schwann Cell Transplantation Methods Using Biomaterials.- Viral Transduction of Schwann Cells for Peripheral Nerve Repair.- Intraspinal Delivery of Schwann Cells for Spinal Cord Injury.