AMPK

Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (a1b1y1).- Visualizing AMPK Drug Binding Sites through Crystallization of Full Length Phosphorylated a2b1y1 Heterotrimer.- Biophysical Interactions of Direct AMPK Activators.- Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship.- Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK.- Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate Binding Modules.- Bioinformatics Approach to Identify Novel AMPK Targets.- Studying AMPK in an Evolutionary Context.- AMPK Protein Interaction Analyses by Yeast Two-Hybrid.- Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells.- Knockdown of Human AMPK using the CRISPR-Cas9 Genome-Editing System.- Compound C/Dorsomorphin – Its Use and Misuse as an AMPK Inhibitor.- Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscleby Immunoprecipitation.- Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in Skeletal Muscle.- Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High Performance Liquid Chromatography.- Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation.- Cellular Application of Genetically-Encoded Sensors and Impeders of AMPK.- Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles.- Study of AMPK Regulated Metabolic Fluxes in Neurons using the Seahorse XFe Analyzer.- Investigating the Role of AMPK in Inflammation.- Studying the Role of AMPK in Cardiac Hypertrophy and Protein Synthesis.- Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake.- Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured Cells.- Studying the Role of AMPK in Autophagy.- Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1Axis.- Manipulation and Measurement of AMPK Activity in Pancreatic Islets.- Analyzing AMPK Function in the Hypothalamus.- Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney Proteins.- A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability upon Metabolic Stress.- Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis.- Modulation of Vascular Function by AMPK: Assessment of NO Bioavailability and Surrogates of Oxidative Stress.- Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood Vessels.- Studying the Role of AMPK in Angiogenesis.- Analysis of Muscle Stem Cell Fate through Modulation of AMPK Activity.- Evaluating the Role of Host AMPK in Leishmania Burden.- Analysis of Transgenerational Phenotypes Following Acute Atarvation in AMPK-Deficient C. elegans.- Human γ2 AMPK Mutations.