Plant Cell Culture Protocols

An Introduction to Plant Cell Culture.- History of Plant Tissue Culture.- Callus, Suspension Culture and Hairy Roots. Induction, Maintenance, and Characterization.- Growth Measurements. Estimation of Cell Division and Cell Expansion.- Measurement of Cell Viability.- Pathogen and Biological Contamination Management in Plant Tissue Culture: Phyto-Pathogens, Vitro-Pathogens, and Vitro-Pests.- Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification and Encapsulation-Dehydration.- The Study of in vitro Development in Plants: General Approaches and Photography.- Use of Statistics in Plant Biotechnology.- Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela Odorata).- Micropropagation of Banana.- Liquid in vitro Culture for the Propagation of Arundo donax.- Production of Haploids and Doubled Haploids in Maize.- Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration.- Improved Shoot Regeneration from Root Explants Using an Abscisic Acid Containing Medium.- Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies.- Anther Culture of Chili Pepper (Capsicum spp.).- Production of Inter-Specific Hybrids in Ornamental Plants.- Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research.- Removing Heavy Metals by in vitro Cultures.- Establishment of a Sanguinarine Producing Cell Suspension Culture of Argemone Mexicana  L. (Papaveraceae). Induction of  Alkaloid Accumulation.- Epigenetics, the Role of DNA Methylation on Tree Development.- The Potential Roles of microRNAs in Molecular Breeding.- Determination of Histone Methylation in Mono- and Dicotyledons Plants.- Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture.- Plant Tissue Culture and Molecular Markers.- Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat.- Improved Genetic Transformation of Cork Oak (Quercus suber L.).- OrganelleTransformation.- Appendix A - The Components of the Culture Media.- Appendix B - Plant Biotechnology and Tissue Culture Resources in the Internet.