The Giant Vesicle Book

Rumiana Dimova leads an experimental lab in biophysics at the Max Planck Institute of Colloids and Interfaces in Potsdam, Germany. She has been working with giant vesicles already from the beginning of her scientific career. After being introduced into the magic of their preparation during her studies as a student in Bulgaria, she remained fascinated by their application and over the years pursued a variety of projects employing giant vesicles as a platform to develop new methods for the biophysical characterization of membranes and processes involving them. Until now, these studies have resulted in more than hundred peer-reviewed publications. Recently, she was also awarded the Emmy Noether distinction for women in physics of the European Physical Society. Carlos Marques, a CNRS senior scientist, founded the MCube group at the Charles Sadron Institute in Strasbourg, France, where he gears experimental and theoretical research towards the understanding of the physical properties of self-assembled lipid bilayers. Trained as a polymer theoretician, Carlos first got interested in membranes because they interact with polymers and published the first prediction for the membrane changes expected when polymers adsorb on lipid bilayers. He then expanded the scope of his group to include experiments and numerical simulations, and has now published many papers based on research with giant unilamellar vesicles, including the first study of lipid oxidation in GUVs and the discovery of the so-called PVA method for vesicle growth.

Preparation methods for giant unilamellar vesicles. Preparation and properties of giant plasma membrane vesicles and giant unilamellar vesicles from natural membranes. Protein reconstitution in giant vesicles. GUVs with cytoskeleton. Understanding giant vesicles – a theoretical perspective. Simulating membranes, vesicles, and cells. Theory of vesicle dynamics in flow and electric fields. Particle-membrane interactions. Theory of polymer-membrane interactions. Application of optical microscopy techniques on giant unilamellar vesicles. Mechanics assays of synthetic lipid membranes based on micropipette aspiration. Atomic force microscopy of giant unilamellar vesicles. Manipulation and biophysical characterization of GUVs with an optical stretcher. Vesicle fluctuation analysis. Using electric fields to assess membrane material properties in GUVs. Creating membrane nanotubes from GUVs. Measuring GUV adhesion. Phase diagrams and tie lines in GUVs. Vesicle dynamics in flow: an experimental approach. Membrane permeability measurements. Lipid and protein mobility in GUVs. Shining light on membranes. Protein-membrane interactions. Effects of antimicrobial peptides and detergents on guvs. Lipid-polymer interactions: effect on GUVs shapes and behavior. Polymersomes. Giant hybrid polymer/lipid vesicles. Giant unilamellar vesicles: from protocell models to the construction of minimal cells. Encapsulation of aqueous two-phase systems and gels within giant lipid vesicles. Droplet-supported giant lipid vesicles as compartments for synthetic biology. List of lipids and physical constants of lipid bilayers. List of membrane dyes and fluorescent groups conjugated to lipids. List of detergents. List of water-soluble dyes or their fluorescent groups and their structures