Plant Receptor Kinases

Expression of Plant Receptor Kinases in E. coli.- Expression of Plant Receptor Kinases in Yeast.- Expression of Plant Receptor Kinases in Tobacco BY-2 Cells.- Cell-free Synthesis of Plant Receptor Kinases.- Purification of Plant Receptor Kinases from Plant Plasma Membranes.- Photoaffinity Labeling of Plant Receptor Kinases.- Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases.- Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis.- In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping.- Co-immunoprecipitation of Plant Receptor Kinases.- Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems.- Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners.- In vitro Assessment of Guanylyl Cyclase Activity ofPlant Receptor Kinases.- BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases.- Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases.- The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases.- Detection and Analyses of Endocytosis of Plant Receptor Kinases.