Biology of Proteoglycans

Preface
Monoclonal Antibodies as Probes for Elucidating Proteoglycan Structure and Function
I. Introduction
II. Production, Screening, and Characterization of Monoclonal Antibodies Directed against Epitopes Present on Proteoglycan Substructures
III. Monoclonal Antibodies to Epitopes Present on Proteoglycan Monomers
IV. Monoclonal Antibodies to the Link Proteins of Cartilage Proteoglycan Aggregate
V. General Discussion
References
Molecular Biology of Proteoglycans and Link Proteins
I. Perspectives
IL Cell-Free Translation of mRNAs Encoding Proteoglycan Core Proteins and Link Proteins
III. Cloning of Proteoglycan Core Protein and Link Protein cDNAs
IV. Prospectives
References
Biosynthesis of Heparin and Heparan Sulfate
I. Introduction
II. The Proteoglycans—Structures and Interactions
III. Outline of the Biosynthetic Process
IV. Regulation of Polymer Modification
V. Organization of the Biosynthetic Apparatus
VI. Perspectives for the Future
References
Proteoglycan Metabolism by Rat Ovarian Granulosa Cells In Vitro
I. Introduction
II. Characterization of Granulosa Cell Proteoglycans
III. Metabolism of Granulosa Cell Proteoglycans
IV. General Considerations
References
Integral Membrane Proteoglycans as Matrix Receptors: Role in Cytoskeleton and Matrix Assembly at the Epithelial Cell Surface
I. Introduction
II. Characteristics of Mammary Epithelial Cell Surface Proteoglycan
III. Distribution of Epithelial Cell Surface Proteoglycan
IV. Heparan Sulfate Proteoglycan Binds Stromal Matrix Components
V. Cell Surface Proteoglycan as a Matrix Anchor to the Cytoskeleton
VI. Cell Surface Proteoglycan is a Matrix Receptor
VII. Summary
References
Structural Organization of Proteoglycans in Cartilage
I. Introduction
II. Macromolecular Structure of Isolated Proteoglycans Based on Electron-Microscopic Analysis
III. Preservation Methods for Proteoglycans in Tissue
IV. Morphology of Proteoglycans in Cartilage
V. Spatial Arrangement of Proteoglycans in Native Tissue
References
Proteoglycans, Chondrocalcin, and the Calcification of Cartilage Matrix in Endochondrial Ossification
I. Anatomical Organization of the Growth Plate
II. Composition and Organization of the Cartilage Matrix of Growth Plate
III. Changes in the Proteoglycans of the Growth Plate Associated with Calcification
IV. Purified Proteoglycans and Glycosaminoglycans Can Bind Calcium and Inhibit Calcification In Vitro
V. A Unifying Hypothesis to Explain the Proteoglycan Changes That May Occur in Calcifying Growth Plate Cartilage
References
Biochemical Basis of Age-Related Changes in Proteoglycans
I. Introduction
II. Aging and Maturation
III. Proteoglycan Populations: Age-Related Changes
IV. Biosynthesis
V. Proteoglycans in Disease
References
Extracellular Matrix Components of the Synapse
I. Introduction
II. ECM Components are Involved in Synaptic Regeneration
III. Axonal Transport of Sulfated Materials
IV. Proteoglycans in Secretory Vesicles
V. A Synaptic Junctional Proteoglycan Related to the Vesicle Form
VI. Purification and Characterization of the ECM Proteoglycan
VII. The Antigenic Determinant of Electric Organ TAP
VIII. Insertion of the Anchorage Protein
IX. Other Components of the ECM at the Synapse
References
Blood Vessel Proteoglycans
I. Introduction
II. Biochemistry of Arterial Proteoglycans
III. Morphology of Arterial Proteoglycans
IV. Proteoglycans in Atherosclerosis
V. Cell Culture Studies
VI. Concluding Remarks
References
Heparan Sulfate Species and Blood Vessel Wall Function
I. Introduction
II. The Role of Heparan Sulfate Species as a Regulator of Coagulation Mechanism Activity
III. Role of Heparan Sulfate as a Modulator of Smooth Muscle Proliferation
References
Cell-Associated Proteoglycans in Human Malignant Melanoma
I. Extracellular Matrix Composition during Tumorigenesis
II. Chondroitin Sulfate Proteoglycans Synthesized by Human Melanoma Cells: Production of Monoclonal Antibodies
III. Biosynthesis and Intracellular Transport of Proteoglycans
IV. Conclusion
References
Intracellular Proteoglycans in Cells of the Immune System
I. Introduction
II. Localization and Characterization of Secretory Granule Proteoglycans in Different Cell Types
III. Speculated Functions of Intracellular Proteoglycans in Immunity and Inflammation
IV. Summary
References
Index