Thermogenic Fat
Humana Press Inc. (Verlag)
978-1-4939-6819-0 (ISBN)
Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.
Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue.- Isolation of Mouse Stromal Vascular Cells for Monolayer Culture.- Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells.- Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes.- Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells.- Isolation of Immune Cells from Adipose Tissue.- Differentiation and Metabolic Interrogation of Human Adipocytes.- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines.- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue.- Genetic Mouse Models: The Powerful Tools to Study Fat Tissues.- Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function.- Bioenergetic Analyses in Adipose Tissue.- Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry.- Isolation and Patch-Clamp of Primary Adipocytes.- In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver.- Identification and Quantification of Human Brown Adipose Tissue.- Designing 3D Adipospheres for Quantitative Metabolic Study.- Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems.- Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.
Erscheinungsdatum | 24.03.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 1566 |
Zusatzinfo | 30 Illustrations, color; 12 Illustrations, black and white; XI, 214 p. 42 illus., 30 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Naturwissenschaften ► Physik / Astronomie ► Thermodynamik | |
Schlagworte | Adipocytes • Beige fat cells • Brown fat cells • Energy metabolism • Systematic metabolism |
ISBN-10 | 1-4939-6819-X / 149396819X |
ISBN-13 | 978-1-4939-6819-0 / 9781493968190 |
Zustand | Neuware |
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