Site-Specific Protein Labeling -

Site-Specific Protein Labeling

Methods and Protocols
Buch | Softcover
267 Seiten
2016 | Softcover reprint of the original 1st ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-4417-0 (ISBN)
117,69 inkl. MwSt
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag.- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery.- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins.- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes.- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues.- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels.- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells.- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins.- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing.- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins.- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling.- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist.- BONCAT – Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes.- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins.- Labeling Proteins By Affinity-Guided DMAP Chemistry.- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1266
Zusatzinfo 34 Illustrations, color; 38 Illustrations, black and white; XII, 267 p. 72 illus., 34 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Schlagworte CLIP-tag technology • FlAsH-tag • intracellular proteins • labeled proteins • membrane translocation • Protein translation • site-specific protein labeling • SNAP-tag • tag-based methods • Unnatural amino acids
ISBN-10 1-4939-4417-7 / 1493944177
ISBN-13 978-1-4939-4417-0 / 9781493944170
Zustand Neuware
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