Site-Specific Protein Labeling
Humana Press Inc. (Verlag)
978-1-4939-4417-0 (ISBN)
Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.
How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag.- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery.- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins.- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes.- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues.- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels.- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells.- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins.- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing.- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins.- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling.- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist.- BONCAT – Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes.- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins.- Labeling Proteins By Affinity-Guided DMAP Chemistry.- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.
Erscheinungsdatum | 23.09.2022 |
---|---|
Reihe/Serie | Methods in Molecular Biology ; 1266 |
Zusatzinfo | 34 Illustrations, color; 38 Illustrations, black and white; XII, 267 p. 72 illus., 34 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | CLIP-tag technology • FlAsH-tag • intracellular proteins • labeled proteins • membrane translocation • Protein translation • site-specific protein labeling • SNAP-tag • tag-based methods • Unnatural amino acids |
ISBN-10 | 1-4939-4417-7 / 1493944177 |
ISBN-13 | 978-1-4939-4417-0 / 9781493944170 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich