Site-Specific Protein Labeling

How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag.- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery.- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins.- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes.- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues.- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels.- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells.- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins.- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing.- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins.- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling.- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist.- BONCAT – Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes.- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins.- Labeling Proteins By Affinity-Guided DMAP Chemistry.- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.