Cell Fusion -

Cell Fusion

Overviews and Methods

Kurt Pfannkuche (Herausgeber)

Buch | Softcover
248 Seiten
2016 | Softcover reprint of the original 2nd ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-4946-5 (ISBN)
101,64 inkl. MwSt
This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo. Additional, protocols aim to facilitate the future highly relevant process of engineering cells for specific purposes, such as generation of transgenic embryos and development of hybrid cells, in order to tackle specific tasks in cell biology and medicine. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Cell Fusion: Overviews and Methods, Second Edition serves as an ideal reference on cell fusion and laboratory protocols.

State of the Art in Cell-Cell Fusion.- Fusion in Cancer: An Explanatory Model for Aneuploidy, Metastasis Formatio, and Drug Resistance.- Mouse Embryos´ Fusion for the Tetraploid Complementation Assay.- Generation of Mouse Chimeras with High Contribution of Tetraploid Embryonic Stem Cells and Embryonic Stem Cell-fibroblast Hybrid Cells.- Microfluidic Systems for Cell Pairing and Fusion.- Chromosome Tracking in Fused Cells by Single Nucleotide Polymorphisms.- Fusion of Mesenchymal Stem Cells and Islet Cells for Cell Therapy.- Detection of Fusion Events in Mammalian Skeletal Muscle.- FISH Detection of X and Y Chromosomes in Combination with Immunofluorescence to Study Contribution of Transplanted Cells to Skeletal Muscle Fibers.- Using Phosphatidylserine Exposure on Apoptotic Cells to Stimulate Myoblast Fusion.- Tracing Myoblast Fusion in Drosophila Embryos by Fluorescent Actin Probes.- Analysing Cell Fusion Events Within the Central Nervous System using Bone Marrow Chimerism.- Cell Fusion Between Dendritic Cells and Whole Tumor Cells.- Membrane Nanotube Formation in Osteoclastogenesis.- Modified Adherence Method (MAM) for Electrofusion of Anchorage Dependent Cells.- FRET in the Analysis of in vitro Cell-cell Fusion by Flow Cytometry.- Dual Split Protein (DSP) Assay to Monitor Cell-cell Membrane Fusion.- Photoconvertible Fluorescent Protein‐based Live Imaging of Mitochondrial Fusion.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1313
Zusatzinfo 30 Illustrations, color; 11 Illustrations, black and white; XI, 248 p. 41 illus., 30 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Biologie Zellbiologie
Schlagworte cell fuse • Cell organelles • engineering cells • hybrid cells • mitochondrial function • mitochondrial fusion • sub-cellular fusion processes • transgenic embryos
ISBN-10 1-4939-4946-2 / 1493949462
ISBN-13 978-1-4939-4946-5 / 9781493949465
Zustand Neuware
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