Membrane Trafficking

Intracellular Parcel Service: Current Issues in Intracellular Membrane Trafficking.- In vitro Analysis of the Mitochondrial Preprotein Import Machinery Using Recombinant Precursor Polypeptides.- Import of Proteins into Isolated Yeast Mitochondria.- Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae.- Fractionation of Plasmodium-infected Human Red Blood Cells to Study Protein Trafficking.- Investigating Signalling Processes in Membrane Trafficking.- Recruitment of Coat Proteins to Liposomes and Peptidoliposomes.- A β-lactamase Based Assay to Measure Surface Expression of Membrane Proteins.- Cell-free Reconstitution of Multivesicular Body (MVB) Cargo Sorting.- The Analysis of the Biogenesis of Lipid Droplets by Examining Rab40c Associating With Lipid Droplets.- Analysis of Conventional and Unconventional Trafficking of CFTR and Other Membrane Proteins.- Assessing Mammalian Autophagy.- Expression of Functional Myc-tagged Conserved Oligomeric Golgi (COG) Subcomplexes in Mammalian Cells.- Molecular and Cellular Characterization of GCC185, a Tethering Protein of the trans-Golgi Network.- Visualizing Toll-like Receptor-dependent Phagosomal Dynamics in Murine Dendritic Cells Using Live Cell Microscopy.- Understanding of Complex Protein Interactions with Respect to Anchorage Independence.- Application of Flow Cytometry to Analyse Intracellular Location and Trafficking of Cargo in Cell Populations.- Approaches to Analyze the Role of Rab GTPases in Endocytic Trafficking of Epidermal Growth Factor Receptor (EGFR).- Does Super Resolution Fluorescence Microscopy Obsolete Previous Microscopic Approaches to Protein Co-localization?.- Bimolecular Fluorescence Complementation (BiFC) Technique in Yeast Saccharomyces cerevisiae and Mammalian Cells.- Microscopic and Spectroscopic Techniques to Investigate Lipid Droplet Formation and Turnover in Yeast.- Image-based Identification of Nuclear Export Inhibitors From Natural Products.-Correlative Video-light-electron Microscopy of Mobile Organelles.- Live Cell Imaging of Endosomal Trafficking in Fungi.- Quantitative Analysis of Transferrin Cycling by Automated Fluorescence Microscopy.- Identification of Factors Regulating MET Receptor Endocytosis by High Throughput siRNA Screening.- Large-scale Analysis of Membrane Transport in Yeast Using Invertase Reporters.- RNAi Screens for Genes Involved in Golgi Glycosylation.- Proteomics Analyses of an Unique Bi-lobed Structure in Trypanosoma brucei.- Application of the Proximity-dependent Assay and Fluorescence Imaging Approaches to Study Viral Entry Pathways.