Yeast Genetics

Yeast Transformation by LiAc/SS Carrier DNA/PEG Method.- Tetrad, Random Spore and Molecular Analysis of Meiotic Segregation and Recombination.- PCR Mutagenesis and Gap Repair in Yeast.- PCR-Mediated Epitope Tagging of Genes in Yeast.- Manipulating the Yeast Genome: Deletion, Mutation and Tagging by PCR.- Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence.- Single Yeast Cell Imaging.- Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells.- Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations.- Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast.- Chemical Genetic and Chemogenomic Analysis in Yeast.- Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays.- Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast.- Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.- Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq.- High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast.- Analysis of Silencing in Saccharomyces cerevisiae.- A User’s Guide to the Ribosomal DANN in Saccharomysces cerevisiae.- Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication.- Replicative Life Span Alaysis in Budding Yeast.- Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast.