The Baculovirus Expression System
A Laboratory Guide
Seiten
1992
Chapman and Hall (Verlag)
978-0-412-37150-9 (ISBN)
Chapman and Hall (Verlag)
978-0-412-37150-9 (ISBN)
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A practical guide covering the background, methodological details, applications, advantages and disadvantages of the use of baculovirus expression systems, designed to teach the first-time user the techniques and to highlight more advanced applications for the experienced user.
As a result of recent major advances in molecular biological techniques, the use of baculovirus expression systems is now widespread and publications reviewing the techniques are numerous. The aim of this practical manual is to give first-time users the relevant background information and detailed laboratory methods. Methods and protocols are provided and more detailed applications are highlighted for the benefit of the more experienced user. Emphasis is placed on practical applications, interpretation of results and potential problems and pitfalls.
As a result of recent major advances in molecular biological techniques, the use of baculovirus expression systems is now widespread and publications reviewing the techniques are numerous. The aim of this practical manual is to give first-time users the relevant background information and detailed laboratory methods. Methods and protocols are provided and more detailed applications are highlighted for the benefit of the more experienced user. Emphasis is placed on practical applications, interpretation of results and potential problems and pitfalls.
The baculoviruses - introduction; the development of baculovirus expression vectors; processing of foreign proteins synthesized using baculovirus vectors in insect cells; construction of transfer vectors containing the foreign gene; insect cell culture media and maintenance of insect cell lines; propagation, titration and purification of AcMNPV in cell culture; production and selelction of recombinant virus; characterization of recombinant viruses; scaling up the production of recombinant proteins in insect cells - laboratory bench level; propagation of baculoviruses in insect larvae.
| Verlagsort | London |
|---|---|
| Sprache | englisch |
| Maße | 156 x 234 mm |
| Gewicht | 470 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-412-37150-2 / 0412371502 |
| ISBN-13 | 978-0-412-37150-9 / 9780412371509 |
| Zustand | Neuware |
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