Legionella
Humana Press Inc. (Verlag)
978-1-4939-6274-7 (ISBN)
Authoritative and easily accessible, Legionella: Methods and Protocols will not only be useful for research groups studying Legionella, but also for a broader scientific community studying the epidemiology, typing, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.
Identification of Legionella in the Environment.- Identification of Legionella in Clinical Samples.- Sequence-Based Identification of Legionella.- Investigation of Outbreaks - Epidemiology.- Outbreak Investigations and Identification of Legionella in Contaminated Water.- Typing Methods for Legionella.- Culture, Media and Handling of Legionella.- The Intracellular Metabolism of Legionella by Isotopologue Profiling.- Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction.- Constructing Unmarked Gene Deletions in Legionella pneumophila.- Natural Biofilm Formation with Legionella pneumophila.- Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium.- Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila.- Analysis of Legionella Infection by Flow Cytometry.- Analysis of Legionella Infection Using RNAi in Drosophila Cells.- Immunofluorescence Imaging of Legionella Infection In vitro.- Electron Microscopy of Legionella and Legionella-Infected Cells.- Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages.- Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System.- Subcellular Localization of Legionella Dot/Icm Effectors.- Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase.- Legionella Phospholipases Implicated in Infection – Determination of Enzymatic Activities.- Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids.- Characterization of Legionella lipopolysaccharide.- The Tetrahymena and Acanthamoeba Model Systems.- Dictyostelium Host Response to Legionella infection: Strategies and Assays.- The Caenorhabditis elegans Model of Legionella Infection.- Infection of Nonphagocytic Host Cells by Legionella.- Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages.- The Mouse as a Model for Pulmonary Legionella Infection.- Assessment of Legionella-Specific Immunity in Mice.- The Guinea Pig Model of Legionnaires’ Disease.- Human Susceptibility to Legionnaires´ Disease.- cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila.- Methods to Study Legionella Transcriptome in vitro and in vivo.- Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction.- Identification of Legionella Effectors UsingBioinformatic Approaches.
Erscheinungsdatum | 24.07.2016 |
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Reihe/Serie | Methods in Molecular Biology ; 954 |
Zusatzinfo | XXVIII, 622 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Arbeits- / Sozial- / Umweltmedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Studium ► Querschnittsbereiche ► Prävention / Gesundheitsförderung | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Geowissenschaften ► Hydrologie / Ozeanografie | |
ISBN-10 | 1-4939-6274-4 / 1493962744 |
ISBN-13 | 978-1-4939-6274-7 / 9781493962747 |
Zustand | Neuware |
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