Legionella

Identification of Legionella in the Environment.- Identification of Legionella in Clinical Samples.- Sequence-Based Identification of Legionella.- Investigation of Outbreaks - Epidemiology.- Outbreak Investigations and Identification of Legionella in Contaminated Water.- Typing Methods for Legionella.- Culture, Media and Handling of Legionella.- The Intracellular Metabolism of Legionella by Isotopologue Profiling.- Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction.- Constructing Unmarked Gene Deletions in Legionella pneumophila.- Natural Biofilm Formation with Legionella pneumophila.- Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium.- Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila.- Analysis of Legionella Infection by Flow Cytometry.- Analysis of Legionella Infection Using RNAi in Drosophila Cells.- Immunofluorescence Imaging of Legionella Infection In vitro.- Electron Microscopy of Legionella and Legionella-Infected Cells.- Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages.- Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System.- Subcellular Localization of Legionella Dot/Icm Effectors.- Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase.- Legionella Phospholipases Implicated in Infection – Determination of Enzymatic Activities.- Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids.- Characterization of Legionella lipopolysaccharide.- The Tetrahymena and Acanthamoeba Model Systems.- Dictyostelium Host Response to Legionella infection: Strategies and Assays.- The Caenorhabditis elegans Model of Legionella Infection.- Infection of Nonphagocytic Host Cells by Legionella.- Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages.- The Mouse as a Model for Pulmonary Legionella Infection.- Assessment of Legionella-Specific Immunity in Mice.- The Guinea Pig Model of Legionnaires’ Disease.- Human Susceptibility to Legionnaires´ Disease.- cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila.- Methods to Study Legionella Transcriptome in vitro and in vivo.- Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction.- Identification of Legionella Effectors UsingBioinformatic Approaches.