Mass Spectrometry of Glycoproteins
Humana Press Inc. (Verlag)
978-1-4939-6203-7 (ISBN)
Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.
Introduction to Glycosylation and Mass Spectrometry.-Tandem Lectin Weak Affinity Chromatography (LWAC) for Glycoprotein Enrichment.-CSC Technology - Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells.-Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment.-Incorporation of Unnatural Sugars for the Identification of Glycoproteins.-Characterization of Membrane-associated Glycoproteins using Lectin Affinity Chromatography and Mass Spectrometry.-Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptides.-In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysis.-Nano-HPLC-MS of Glycopeptides Obtained After Non-specific Proteolysis.-Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE).-Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection.-Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomics.-Quantitative Analysis of Glycoprotein Glycans.-Stable Isotope Labeling of N-glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometry-based Glycoproteomics.-Approaches for Site Mapping and Quantification of O-linked Glycopeptides.-Glycan Profiling: Label-free Analysis of Glycoproteins.-Introduction to Informatics in Glycoprotein Analysis.-Software Tools for Glycan Profiling.-Quantitative Characterization of Glycoproteins in Neurodegenerative Disorders using iTRAQ.-Quantitative Proteomic Analysis of N-linked Glycoproteins in Human Tear Fluid.-Elucidation of N-glycosites Within Human Plasma Glycoproteins for Cancer Biomarker Discovery.-Characterizing the Glycosylation State of Therapeutic RecombinantGlycoproteins.
Erscheinungsdatum | 27.06.2016 |
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Reihe/Serie | Methods in Molecular Biology ; 951 |
Zusatzinfo | XI, 339 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Chemie ► Analytische Chemie | |
ISBN-10 | 1-4939-6203-5 / 1493962035 |
ISBN-13 | 978-1-4939-6203-7 / 9781493962037 |
Zustand | Neuware |
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