Plant Epigenetics
Springer-Verlag New York Inc.
978-1-4899-7706-9 (ISBN)
volume provides a variety of protocols to analyze various epigenetic
changes, including differential expression of non-coding RNAs, changes in DNA
methylation, and histone modifications in plants. Chapters detail protocols
with different degrees of complexity, and describe bioinformatics approaches
for data processing and analysis. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and
cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition
aims to ensure successful results in the further study of this vital field.
Chromatin Immunoprecipitation
Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis.- Chromatin Conformation Capture-Based Analysis of Nuclear Architecture.- Metaanalysis of Genome-Wide Chromatin Data.- Localization of Mirnas by In Situ Hybridization in Plants using Conventional
Oligonucleotide Probes.- COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of
Locus-Specific Changes in Methylation Patterns.- Analysis of Global Genome Methylation Using the Cytosine-Extension Assay.- In Situ Analysis Of DNA Methylation In Plants.- Analysis of DNA Hydroxymethylation In Arabidopsis Using
Colorimetric Assay.- Analysis of DNA Cytosine Methylation Patterns using
Methylation Specific Amplification Polymorphism (MSAP).- Differentially Methylated
Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify
Dmrs in Uncharacterized Genomes.- Analysis of Small RNA PopulationsUsing
Hybridization to DNA Tiling Arrays.- Northern Blotting Techniques for Small RNAs.- Stem-Loop Qrt-PCR for the Detection of Plant
Micrornas.- Profiling New Small RNA Sequences.- Small RNA Library Preparation and Illumina Sequencing in Plants.- Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow.- Increasing
a Stable Transformation Efficiency of Arabidopsis by Manipulating the
Endogenous Gene Expression using Virus-Induced Gene Silencing.- The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA
Strand Breaks.- Profiling Transposable Elements and their Epigenetic Effects in Non-Model
Species.
| Erscheinungsdatum | 22.09.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1456 |
| Zusatzinfo | 32 Illustrations, color; 19 Illustrations, black and white; X, 253 p. 51 illus., 32 illus. in color. |
| Verlagsort | New York |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
| Schlagworte | bioinformatics approaches • DNA Methylation • epigenetic changes • histone modifications in plants • Non-coding RNAs |
| ISBN-10 | 1-4899-7706-6 / 1489977066 |
| ISBN-13 | 978-1-4899-7706-9 / 9781489977069 |
| Zustand | Neuware |
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