Plant Proteostasis

Approaches to Determine Protein Ubiquitination Residue
Types.- Immunoprecipitation of Cullin Ring Ligases
(CRLs) in Arabidopsis Thaliana Seedlings.- Radioligand Binding Assays for Determining Dissociation Constants of
Phytohormone Receptors.- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes.- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants.- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro.- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases.- SUMO Chain
Formation by Plants Enzymes.- Kinetic Analysis of Plant SUMO Conjugation Machinery.- Expression, Purification, and Enzymatic
Analysis Of Plant SUMO Proteases.- Quantitative Analysis of
Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy
Imaging.- Biochemical Analysis of Autophagy in Algae and Plants by Monitoring
the Electrophoretic Mobility of ATG8.- Detection of Autophagy in
Plants by Fluorescence Microscopy.- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System.- Detection and Quantification of Protein Aggregates in Plants.- Determination of Protein
Carbonylation and Proteasome Activity in Seeds.- Isobaric Tag for Relative
and Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate Affinity
Chromatography (PIP - chromatography) for the Isolation of Proteins Involved in
Protein Quality Control and Proteostasis Mechanisms in Plants.- In vivo Radiolabeling of Arabidopsis Leaves and Separation of Thylakoid
Membrane Complexes by Blue Native PAGE.- Normalized Quantitative Western Blotting
Based on Standardized Fluorescent Labeling.- Sequence Search and
Comparative Genomic Analysis of SUMO-Activating Enzymes using CoGe.- Studying Evolutionary Dynamics
of Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest.- Bioinformatics Tools for Exploring the SUMO Gene Network.