Mycobacteria Protocols

Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly.- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis.- RNA Sequencing for Transcript 5’-end Mapping in Mycobacteria.- Fractionation and Analysis of Mycobacterial Proteins.- Lipid and Lipoarabinomannan Isolation and Characterization.- Metabolomics of Mycobacterium tuberculosis.- Electroporation of Mycobacteria.- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination.- Construction of Conditional Knockdown Mutants in Mycobacteria.- Mycobacterial Recombineering.- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria.- Genetic Dissection of Mycobacterial Biofilms.- Measuring Efflux and Permeability in Mycobacteria.- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy.- Antimicrobial Susceptibility Testing for Mycobacterium sp..- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis.- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis.- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis.- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis.- Macrophage Infection Models for Mycobacterium tuberculosis.- Infection of Human Neutrophils to Study Virulence Properties of Mycobacteriumtuberculosis.- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids.- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum.- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.