Mitochondrial Regulation

Isolation of Crud Mitochondrial Fraction from Cells.- Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein – Analyses of MicroRNA and Protein Expressions.- PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species.- NAD+ Content and its Role in Mitochondria.- Measuring PGC-1α and its Acetylation Status in Mouse Primary Myotubes.- Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes.- Determination Of Oxidative Phosphorylation Complexes Activities.- Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects.- A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-resolved Studies.- BN-PAGE-based Approach to Study Thyroid Hormones and Mitochondrial Function.- Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-Phosphorylating Thymus Mitochondria.- Exploring Liver Mitochondrial Function By 13c-Stable Isotope Breath Tests. Implications In Clinical Biochemistry.- Following Mitochondria Dynamism: Confocal Analysis of The Organelle Morphology.- Analysis of Pro-apoptotic Protein Trafficking to and From Mitochondria.- Mitophagy and Mitochondrial Balance.