Antibiotic Resistance Methods and Protocols

Part I. Diagnosis of Resistance. Multiplex Polymerase Chain Reaction Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci, Robin Patel, Jim R. Uhl, and Franklin R. Cockerill, III. Drug Susceptibility of Mycobacterium tuberculosis Through the Mycolic Acid Index, Jose M. Viader-Salvado, Martha Guerrero-Olazaran, Elvira Garzo-Gonzalez, and Rolando Tijerina-Menchaca. Micro-Well Phage Replication Assay for Screening Mycobacteria for Resistance to Rifampin and Streptomycin, Ruth McNerney. Application of SSCP to Identification of Resistance Mutations, Timothy D. McHugh. Quantitative, Single-Tube, Nested, PCR (QSTN-PCR) for Determining the Antibiotic Susceptibility of Mycobacterium tuberculosis, Nainn-Tsyr Jou and Michael R. Liebling. Rapid Rifamycin Susceptibility Testing of Small-Volume Mycobacterium tuberculosis Cultures by Detection of Precursor rRNA, William H. Brabant and Gerard A. Cangelosi. Detection of Penicillin Resistance in Streptococcus pneumoiae by a Seminested PCR Strategy, Mignon du Plessis, Anthony M. Smith, and Keith P. Klugman. Diagnosis of Penicillin Resistance by PCR-RFLP, Gail C. Whiting. Detection Methods of Glycopeptide-Restraint Staphylococcus aureus I: Susceptibility Testing, Hedeaki Hanaki and Keiichi Hiramatsu. Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II: Cell Wall Analysis, Hedeaki Hanaki and Keiichi Hiramatsu. Multiplex PCR for the Rapid Simultaneous Speciation and Detection of Methicillin-Resistance and Genes Encoding Toxin Production in Staphylococcus aureus, Mark E. Jones, Karl Kohrer, and Franz-Josef Schmitz. Chromogenic Detection of Aminoglycoside Phosphotransferases, Ana M. Amoroso and Gabriel O. Gutkind. Part II. Monitoring Response to Treatment. Quantification of M. tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosis, Lucy E. DesJardin and Gery L. Hehman. Isolation of M. tuberculosis RNA from Sputum, Lucy E. DesJardin. Detection of Viable Mycobacterium tuberculosis by Reverse Transcriptase-Strand Displacement of mRNA, Tobin J. Hellyer. Part III. Molecular Epidemiology. BOX PCR Fingerprinting for Molecular Typing of Streptococcus pneumoniae, Alex van Belkum and Peter W. M. Hermans. Restriction Fragment End Labeling Analysis: High-Resolution Genomic Typing of Streptococcus pneumoniae Isolates, Peter W. M. Hermans, Marcel Sluijter, and Alex van Belkum. Pulse-Field Gel Electrophoresis for Epidemiological Studies of Streptococcus pneumoniae, Anne-Marie Gasc. Outer Membrane Profiles of Clonally Related Klebsiella pneumoniae, Vicente J. Benedi and Luis Martinez -Martinez. Atomic Force Microscopy: Theory and Practice in Bacteria Morphostructural Analysis, Pier Carlo Braga and Davide Ricci. Part IV. Biology of Resistance. Assessing the Activity of Bacterial Multidrug Efflux Pumps, Keith Poole and Ramakrishnan Srikumar. The Use of a Continuous Culture System to Study the Antimicrobial Susceptibility of Bacteria in Biofilm, J. Keith Struthers. Estimation of Mutation Rates in Antibiotic Research, Owen J. Billington and Stephen H. Gillespie. In Vitro Assessment of the Fitness of Resistant M. tuberculosis Bacteria by Competition Assay, Stephen H. Gillespie. Purification of DNA Topoisomerases and Inhibition by Fluoroqinolones, John T. George and Ian Morrissey. Site-Directed Mutagenesis to Determine Structure Function Relationships in Streptococcus pneumoniae Penicillin-Binding Protein Genes, Victoria A. Barcus and Christopher G. Dowson. Detection of Low Affinity Penicillin-Binding Protein Variants in Streptococcus pneumoniae, Regine Hakenbeck. Part V. Transmission of Resistance. Mobilization of Transposons: Rationale and Techniques for Detection, Louis B. Rice. Index.