Genotoxicity Assessment

Methods and Protocols

Alok Dhawan, Mahima Bajpayee (Herausgeber)

Buch | Hardcover
463 Seiten
2013
Humana Press Inc. (Verlag)
978-1-62703-528-6 (ISBN)

Lese- und Medienproben

Genotoxicity Assessment -
181,89 inkl. MwSt
This Methods in Molecular Biology book offers in vitro and in vivo protocols on mutation assays, cytogenetic techniques, primary DNA damage, alternate assays to animal models and updated ICH guidelines. Includes material lists, troubleshooting tips and more.
Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.

Bacterial Mutation Assays.- In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay.- Erythrocyte--Based Pig--a Gene Mutation Assay.- Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes.- In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and Rats.- In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests.- Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse.- Chromosomal Aberration Test in Human Lymphocytes.- In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood.- Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells.- Flow Cytometric Determination of Micronucleus Frequency.- Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test.- Comparative Genomic Hybridization (CGH) in Genotoxicology.- The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity.- g--H2AX Detection in Somatic and Germ Cells of Mice.- Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers.- The Comet Assay: Assessment of in vitro and in vivo DNA Damage.- The Comet Assay in Human Biomonitoring.- The Comet assay in Marine Animals.- Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo.- 32P--Postlabeling Analysis of DNA Adducts.- Micronucleus Assay with Tetrad Cells of Tradescantia.- The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila.- Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH).

Reihe/Serie Methods in Molecular Biology ; 1044
Zusatzinfo 62 Illustrations, color; 44 Illustrations, black and white; XV, 463 p. 106 illus., 62 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Pharmakologie / Pharmakotherapie
Medizin / Pharmazie Pharmazie
Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Genetik / Molekularbiologie
Schlagworte Comet assay technique • cytogenetic techniques • DNA • DNA synthesis and 32P--post labelling • Drosophila • Genotoxicity Assessment • genotoxicity assessment of chemicals • ICH guidelines • in vitro in bacteria • mouse lymphoma • primary DNA damage • test--Pig--a gene mutation • Transgenic animals
ISBN-10 1-62703-528-1 / 1627035281
ISBN-13 978-1-62703-528-6 / 9781627035286
Zustand Neuware
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