Advanced Techniques in Biological Electron Microscopy III

Three-Dimensional Reconstruction of Nonperiodic Macromolecular Assemblies from Electron Micrographs.- 1 Introduction.- 2 Methods for Obtaining Projection Data.- 3 Methods of Three-Dimensional Reconstruction.- 4 Experimental Results.- 5 Conclusions.- References.- High Resolution Biological X-Ray Microanalysis of Diffusable Ions.- 1 Introduction.- 2 Principles of the Technique.- 3 Specimen Preparation.- 4 Quantitation.- 5 Applications in the Analysis of Diffusable Ions.- 6 Conclusion.- References.- Metal Deposition by High-Energy Sputtering for High Magnification Electron Microscopy.- 1 Introduction.- 2 Methods.- 3 Metal Deposition.- 4 Conclusion.- References.- Computer Programs for Biological Stereology.- 1 Introduction.- 2 Point Counting Programs.- 3 Digitizing Programs.- 4 Special Purpose Stereology Programs.- 5 Concluding Comments.- References.- A Guide to Fracture Label: Cytochemical Labeling of Freeze-Fractured Cells.- 1 Introduction.- 2 Experimental Procedures.- 3 Electron Microscopy.- 4 Interpretation.- References.- The Preparation of Colloidal Gold Probes and Their Use as Marker in Electron Microscopy.- 1 Introduction.- 2 The Preparation and Storage of Colloidal Gold Sols.- 3 The Production, Purification, and Storage of Gold Probes.- 4 Quality Control and Analysis of Gold Sols and Probes.- 5 Critical Evaluation of the Use of Gold Probes in Selected Marking Techniques.- 6 Conclusions.- References.