Angiogenesis Assays (eBook)
410 Seiten
John Wiley & Sons (Verlag)
978-0-470-02934-3 (ISBN)
existing vasculature, is essential for physiological growth and
over 18,000 research articles have been published describing the
role of angiogenesis in over 70 different diseases, including
cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis.
One of the most important technical challenges in such studies has
been finding suitable methods for assessing the effects of
regulators of eh angiogenic response. While increasing numbers of
angiogenesis assays are being described both in vitro and in vivo,
it is often still necessary to use a combination of assays to
identify the cellular and molecular events in angiogenesis and the
full range of effects of a given test protein. Although the
endothelial cell - its migration, proliferation, differentiation
and structural rearrangement - is central to the angiogenic
process, it is not the only cell type involved. the supporting
cells, the extracellular matrix and the circulating blood with its
cellular and humoral components also contribute.
In this book, experts in the use of a diverse range of assays
outline key components of these and give a critical appraisal of
their strengths and weaknesses. Examples include assays for the
proliferation, migration and differentiation of endothelial cells
in vitro, vessel outgrowth from organ cultures, assessment of
endothelial and mural cell interactions, and such in vivo assays as
the chick chorioallantoic membrane, zebrafish, corneal, chamber and
tumour angiogenesis models. These are followed by a critical
analysis of the biological end-points currently being used in
clinical trials to assess the clinical efficacy of anti-angiogenic
drugs, which leads into a discussion of the direction future
studies should take.
This valuable book is of interest to research scientists
currently working on angiogenesis in both the academic community
and in the biotechnology and pharmaceutical industries. Relevant
disciplines include cell and molecular biology, oncology,
cardiovascular research, biotechnology, pharmacology, pathology and
physiology.
Dr CA Staton. Lecturer in Angiogenesis Research, Academic Unit of Surgical Oncology, University of Sheffield Medical School. Professor Claire Lewis. Professor of Molecular & Cellular Pathology, Academic Unit of Pathology, University of Sheffield Medical School. Professor Roy Bicknell. CRUK Molecular Angiogenesis Labs, Institute of Molecular Medicine, University of Oxford.
Preface.
List of Contributors.
1 Endothelial cell biology(Femke Hillen, Veerle
Melotte, Judy R. van Beijnum and Arjan W. Griffioen).
2 Endothelial cell proliferation assays (Wen-Sen
Lee).
3 Endothelial cell migration assays (Christos
Polytarchou, Maria Hatziapostolou and Evangelia
Papadimitriou).
4 Tubule formation assays (Ewen J. Smith and Carolyn
A. Staton).
5 Modelling the effects of the haemodynamic environment on
endothelial cell responses relevant to angiogenesis (Gerard
B. Nash and Stuart Egginton).
6 Whole or partial vessel outgrowth assays (Cindy H.
Chau and William D. Figg).
7 Assaying endothelial-mural cell interactions
(Melissa K. Nix and Karen K. Hirschi).
8 Assays for membrane and intracellular signalling events
(Vittorio Tomasi, Cristiana Griffoni, Spartaco Santi, Patrizia
Lenaz, Rosa Anna Iorio, Antonio Strillaci and Enzo Spisni).
9 Implantation of sponges and polymers (Silvia P.
Andrade, Monica A. N. D. Ferreira and Tai-Ping Fan).
10 Angiogenesis assays in the chick (Andries Zijlstra,
David Mikolon and Dwayne G. Stupack).
11 Corneal angiogenesis assay (Siqing Shan and Mark W.
Dewhirst).
12 Dorsal air sac model (Sei Yonezawa, Tomohiro Asai
and Naoto Oku).
13 Chamber assays (Michael D. Menger, Matthias W.
Laschke and Brigitte Vollmar).
14 Tumour models: analysis of angiogenesis in vivo
(Sven A. Lang, M.D. and Oliver Stoeltzing, M.D).
15 Angiomouse: imageable models of angiogenesis
(Robert M. Hoffman).
name="OLE_LINK4">16 Techniques and advances in vascular imaging
in Danio rerio (Kenna R. Mills Shaw and Brant. M.
Weinstein).
17 Biological and clinical implications of recruitment
of stem cells into angiogenesis (Gianluigi Castoldi, Antonio
Cuneo and Gian Matteo Rigolin).
18 Methods for monitoring of the anti-angiogenic activity of
agents in patients: novel trial design (Shannon Smiley,
Michael K. K. Wong and Shaker A. Mousa).
19 An overview of current angiogenesis assays: Choice of
assay, precautions in interpretation, future requirements and
directions (Robert Auerbach).
Index.
| Erscheint lt. Verlag | 11.1.2007 |
|---|---|
| Sprache | englisch |
| Themenwelt | Medizinische Fachgebiete ► Innere Medizin ► Kardiologie / Angiologie |
| Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Schlagworte | Angiogenese • Biowissenschaften • Cardiovascular Disease • Chemie • Chemistry • endocrinology • Endokrinologie • Kardiovaskuläre Erkrankungen • Kardiovaskuläre Erkrankungen • Life Sciences • Medical Science • Medizin • Onkologie • Pharmaceutical & Medicinal Chemistry • Pharmazeutische u. Medizinische Chemie • structural biology • Strukturbiologie |
| ISBN-10 | 0-470-02934-X / 047002934X |
| ISBN-13 | 978-0-470-02934-3 / 9780470029343 |
| Haben Sie eine Frage zum Produkt? |
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